Male infertility /Azospermia
Female infertility /polycystic ovary/anovulatory/endometrial insufficiency
1. Male infertility /Azospermia </b>
About 15% of couples are infertile with 1 in 3 cases being due to male infertility. Testes produce androgens and sperm formation. Spermatogonial stem cells differentiate into mature spermatozoa in seminiferous tubules of testes.
Ultrasonographic guided intratesticular injection of stem cells
Bone marrow and adipose derived stem cells have potential capabilities to replenish germ cells, supporting cells and testosterone producing cells hence when injected into seminiferous tubules or interstitial spaces. Bone marrow and adipose stem cells were not only able to transdifferentiate into germ cells (spermatogonia and spermatocytes) but as well as Sertoli and Leydig.
How it is done
Patients are evaluated for serum levels of gonadotrophins, testosterone and prolactine
infectious genital disease, and anatomical abnormalities of the genital tract as these issues if present need to be corrected first.
Patients advised to strictly avoid smoking, drug or alcohol consumption
As a day care procedure 240 ml of Bone marrow and 100 ml of fat is aspirated and processed for stem cells isolation. 2 ml of stem cells processed according to GMP is injected either into *Intratesticular
* into the feeding artery of testis
Angiographic guided administration of stem cells in feeding artery of testis
What to expect
Appearance of normal healthy sperm cells at semen analysis is seen by 6 months in 60 %.
*These sperms have Normal activity and possess ability to fertilise ova (validated in lab)
* Fine needle aspiration (FNA) cytology reveals evidence of differentiation and Normal intra testicular spermatogenesis
80 % Patients with oligospermia report significant increase (10 to 20 times) in sperm counts after 3 months of stem cell application
2. Female infertility /polycystic ovary/anovulatory/endometrial insufficiency
There are many biological causes of infertility, including some that medical intervention can treat. Situations exist where multiple IVF had not worked, for reasons that cannot be elucidated by even the most sophisticated diagnostic methods and testing. Two main aetiologies of Female infertility can be successfully ovarian and endometrial rejuvenation via stem cells application has shown a great hope.
1. Anovulatory: lack of ova (anovualtory cycles) can be primary or secondary to diseases like polycystic ovary etc
Ovarian rejuvenation & stimulation via transvaginal administration of stem cells makes the local microenvironment in ovaries favourable thus helps in production of healthy and functional ova
Though the hypothesis exists that these pluripotent stem cells can differentiation into oocytes and could generate new egg cells BUT we believe that as of now this looks unlikely .further it has been seen that ova produced via this mechanism not functional
2. Endometrial causes: leading to infertility are either nonspecific or secondary to diseases like Asherman syndrome, tuberculosis multiple abortions, D&C etc
MSCs tend to secrete antifibrotic, angiogenic, antiapoptotic, immune suppressive factors in a paracrine manner which promote the regeneration of the endometrial lining of the uterus
Stem cell application improves endometrial microenvironment( Endometrial rejuvenation) hence contributing towards promoting fertility and pregnancy in women esp. in Patients in whom the endometrial growth of the cells is poor even in response to the hormonal treatment,
How it is done
Patients are evaluated for hormone levels and prolactine .infectious genital disease, anatomical abnormalities of the genital tract as these issues if present need to be corrected first.
Patients advised to strictly avoid smoking, drug or alcohol consumption and have appositive life style
As a day care procedure 240 ml of Bone marrow and 100 ml of fat is aspirated and processed for stem cells isolation. 2 ml of stem cells processed as per GMP is injected either into
* Trans vaginal administration of cells in ovaries
* Into the feeding artery of ovaries and uterus
* Subendromertial administration in uterus
##along with multiple flushing of uterine wall with growth factors and platelet rich plasma
Ultrasonographic guided transvaginal administration of cells into ovaries
What to expect
55 percent of women have shown evidence of live pregnancy within 6 to 9 months after undergoing the complete treatment protocol.
The endometrium grows up to 7 mm and pregnancy is achieved the embryo transfer in 50 % of caes .
90 % of patients experienced regular menstruation after the stem cell application